Figure 5

Collagenase activity in breast cancer conditioned media Breast cancer cells were cultured for 24 h in serum-free medium in the presence and absence of 2 μg/ml of human plasminogen and TGFβ (10^-10 M). Conditioned media were collected and incubated for 4 h with FITC-labelled type I collagen to detect collagenase activity, as described in Material and methods. The data are expressed as means SEM of 4–6 independent experiments, significantly different from control (**, P < 0.01; *** P < 0.001).