5A. E-cadherin distribution in SUM 149 and SUM 102 human breast cancer cells. 5B. E-cadherin protein expression levels in the IBC cell lineSUM 149 versus SUM 102. 5A. Cells were grown to 60% confluency and stained with anti-E-cadherin (green). Arrows indicate cell-cell adhesions containing E-cadherin. Micrographs were taken at 1000× magnification. Images are representative for at least three independent experiments. 5B. Cell lysates were separated by 8% SDS-PAGE, transferred to nitrocellulose, and probed for E-cadherin. Images are representative of at least three independent experiments with actin serving to verify equal protein loading.