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Figure 4 | Cancer Cell International

Figure 4

From: Dietary omega-3 fatty acids and ionizing irradiation on human breast cancer xenograft growth and angiogenesis

Figure 4

Photomicrographs illustrating the pattern of the tumor vascular network. A, B and C illustrate endothelial pseudopods stained with PAS, D illustrates pseudopods stained with CD-31, a specific endothelial marker and E and F illustrate the localization of HIF. (A) The tumor capsule (left) reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the right has more pseudopods. (B) The subcortical area of the tumor reveals a small blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. (C) At higher magnification endothelial pseudopods are seen to branch. (D) The endothelial pseudopods react positively to the CD-31 specific endothelial marker. (E) Viable cell area can be seen beneath tumor capsule (left). Necrotic area can be seen to the right. (F) Enlarged subcortical area from E. In the subcortex, the brown stain localizes HIF in the hypoxic area between the viable and the necrotic tissue.

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