Figure 1

Photomicrographs illustrating the pattern of the tumor vascularization. A, The tumor capsule (left) of this PAS stained section reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the top right has more pseudopods. B, at higher magnification the subcortical area of the tumor reveals a blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. C, Endothelial pseudopods are seen to branch and occasionally have a vacuole/lumen (arrow). D, The endothelial pseudopods react immunohistochemically positive for the CD-31 specific endothelial cell marker, using the avidin-biotin peroxidase complex method. E, Viable cell area can be seen beneath tumor capsule (left) while necrotic area can be seen to the right. F, Enlarged subcortical area from E. In E, the HIF-α positive area between the viable and the necrotic tissue is stained brown.