Figure 2
Inhibition of focus formation by PC3 cell line stably expressing U94 protein. PC3 cell line transfected with plasmid containing U94 DNA was used for studies. The controls were PC3 cell line transfected with vector cassette or parental PC3 cell line. 1 × 106 cells/ 75 cm2 culture flask was grown to confluence, and focus formation was examined 10 days after. Panel A: PC3 cells (negative control); Panel B: PC3 cells transfected with vector cassette (vector control); Panel C: PC3 cells stably expressing U94 protein (test). Control cells formed foci (Panel A and Panel B) consisting of rounded refractive cells piling on top of each other. Notice that the expression of U94 protein (Panel C) inhibited focus formation. (Magnification: 20X on Olympus CK2 microscope, Olympus Optical Co. Ltd. Japan).