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Figure 3 | Cancer Cell International

Figure 3

From: U94 alters FN1 and ANGPTL4 gene expression and inhibits tumorigenesis of prostate cancer cell line PC3

Figure 3

Tumorigenicity of U94 recombinant PC3 cell line in nude mice. Stable G418 resistant cell lines were generated by transfection of PC3 cells with either pRc-RSV (vector control) or pRc-U94 (test). Confluent cells (5 × 106 cells/100 μl), in culture medium without antibiotics or serum were inoculated behind the neck into athymic nude mice (Ncr nu/nu), and monitored for tumor production. Tumor size was measured on days 7, 14, 17, 21 and 28 post inoculation. Data from two animal experiments (experiment 1: n = 3 per group; and experiment 2, n = 4 per group) were pooled. The average tumor volume in cubic centimeters was plotted against time in days. The error bars represent standard deviation. Notice the significant reduction in tumor size in animals inoculated with U94 recombinant PC3 cell line. A repeated measures analysis of variance demonstrated a significant difference (P < 0.05) in tumor volume between test and control animals. Additionally, paired Student's t-test showed a significant difference in average tumor size of test animals in comparison to control animals.

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