Skip to main content

Table 2 Fold changes of differentially expressed genes in PC3 cell line stablyexpressing U94.

From: U94 alters FN1 and ANGPTL4 gene expression and inhibits tumorigenesis of prostate cancer cell line PC3

PC3/U94 Clones

FNI

SERPINE2

ADAMTS1

ANGPTL4

SPUVE23

   

Microarray

  

1 & 2

91 ± 16

7 ± 1

7 ± 2

-20 ± 4

-10 ± 1

   

QRT-PCR

  

1

183 ± 27

3 ± 1

4 ± 1

-76 ± 13

-6 ± 1

2

467 ± 33

7

10 ± 1

-67 ± 5

-9

  1. Differential expression of genes was determined by microarray, and QRT-PCR was used to confirm microarray data. Microarray data were computed from array analyses presented in Figures 4 and 5. For QRT-PCR, a subculture of PC3 cell lines used for microarray was used for studies: 2 clones of U94 transfected PC3 cells and vector transfected PC3 cells. The protocol for total RNA extraction was same as for microarray studies, except that the RNA extracts were treated with DNase 1. QRT-PCR was performed in triplicate, using SYBR® Green I chemistry, on 7900 HTS Sequence Detection System (Applied Biosystems, Foster City, CA) according to manufacturer's instructions. The results are presented as average of 3 experiments ± SD. Notice that Microarray data and QRT-PCR data show similar trends; moreover the trend of QRT-PCR results was reproducible in 2 clones of U94 recombinant PC3 cell lines.