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Table 1 Amino acid sequences of Pep27 derived from S. pneumoniae and its analogue peptides, and their retention times

From: Functional and structural characteristics of anticancer peptide Pep27 analogues

Peptide

Amino acid sequences

Remarks (substitution)

RT (min)

Pep27

MRKEFHNVLSSGQLLADKRPARDYNRK

(2R→W), (4E→W)

14.54

Pep27anal1

MWKWFHNVLSSWQLLADKRPARDYNRK

(2R→W), (4E→W)

18.67

Pep27anal2

MWKWFHNVLSWWWLLADKRPARDYNRK

(2R→W), (4E→W), (11S→W), (13Q→W)

22.50

Pep27anal3

MRKWFHNVLSSGQLLADKWPAWDYNRK

(4E→W), (19R→W), (22R→W), (26R→W)

20.08

Pep27anal4

MWKEFHNVLSSGQLLADKRWARWYNRW

(2R→W), (20P→W), (23D→W), (27K→W)

19.15

Pep27anal5

MWKWFHNVLSSGQLLADKWWAWWYNWW

(2R→W), (4E→W), (19R→W), (20P→W), (22R→W), (23D→W), (26R→W), (27K→W)

19.17

  1. RT, retention time obtained by RP-HPLC