Figure 2

LiCl treatment of ROS-PG13 cells leads to activation of alkaline phosphatase expression. Protein lysates were prepared from cells treated with 10 mM LiCl or 10 mM NaCl and harvested after the times indicated. Equal amounts of protein extracts were used to measure alkaline phosphatase (AP) activity using p-nitro phenol phosphate as substrate at 420 nm and the relative activity of LiCl treated samples to NaCl treated controls is shown. The experiment represents mean ± SEM. n = 3 per treatment group.