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Table 2 Comparison of tumor characteristics in different regions is shown in pre-drug treated tumor@ and post-treated tumor# by sodium MRI image intensity and histology, histo-immunology parameters as shown in Figures 5 and 6. By using eyepiece-micrometer square counter (100 squares per high power field), necrosis*(<25% cells in micrometer square), viable cells**(<60% cells in micrometer square) and apoptosis*** (20–40 apoptotic nuclei in HPF) and cyst space****(<100 μm) per HPF were premalignant histology characteristics. IC/EC space (% space in HPF), necrosis, viable cells are shown as number of micrometer squares with <25% necrosis area in HPF by histology. Apoptotic index (A.I.) and proliferation index (P.I.) are shown as average number of apoptotic nuclei per HPF and number of mitotic figures per HPF. S-DNA histogram area was measured by CAS 200 system in arbitrary units. Single strand-DNA mAb area was measured in digital images by Optimas 6.5 and ss-DNA mAb density was measured in arbitrary units of photomultiplier scanner.

From: Rapid in vivo Taxotere quantitative chemosensitivity response by 4.23 Tesla sodium MRI and histo-immunostaining features in N-Methyl-N-Nitrosourea induced breast tumors in rats

Pre-drug treated@ Post-drug treated#

Sodium MRI signal intensityd

Histology (in HPF)

A.I. (KI-67)

P.I. (in HPF)

S-DNA (histogram)

ss-DNA mAb (density units)

Tumor feature

IR

SQ

(CAS)

Control (n = 41)

4.25 ± 1.0

5.27 ± 2.0

-

-

-

-

-

Sham (n = 40)

4.30 ± 0.2

5.38 ± 1.0

     

Tumor area(mm2;n = 16)@

4.56 ± 0.30

4.67 ± 0.30

4.49 ± 0.2

-

-

3.6 ± 0.2

4.2 ± 0.2

Tumor area(mm2;n = 3)#

4.60 ± 0.29

4.61 ± 0.26

4.42 ± 0.3

-

-

3.5 ± 0.3

4.3 ± 0.3

IC/EC space@

60–70

 

65–76

-

-

-

-

IC/EC space#

80–95

 

60–70

-

-

-

-

Necrosis*(squares)@

gray

 

56 ± 32

-

270

M-DNA

-

Necrosis*(squares)#

bright

 

50 ± 23

-

120

M-DNA

-

Viable**(squares)@

dark

 

65 ± 21

-

-

-

-

Viable**(squares)#

dark

 

76 ± 11

-

-

-

-

Apoptosis***(nuclei)@

bright

 

40 ± 12

150

-

S-DNA

159 ± 12

Apoptosis***(nuclei)#

bright

 

50 ± 13

160

-

M or S-DNA

129 ± 20

Cyst****(size in μm)@

gray

 

120 ± 29

-

-

-

-

Cyst****(size in μm)#

gray

 

80 ± 22

-

-

-

-