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Table 2 Comparison of tumor characteristics in different regions is shown in pre-drug treated tumor@ and post-treated tumor# by sodium MRI image intensity and histology, histo-immunology parameters as shown in Figures 5 and 6. By using eyepiece-micrometer square counter (100 squares per high power field), necrosis*(<25% cells in micrometer square), viable cells**(<60% cells in micrometer square) and apoptosis*** (20–40 apoptotic nuclei in HPF) and cyst space****(<100 μm) per HPF were premalignant histology characteristics. IC/EC space (% space in HPF), necrosis, viable cells are shown as number of micrometer squares with <25% necrosis area in HPF by histology. Apoptotic index (A.I.) and proliferation index (P.I.) are shown as average number of apoptotic nuclei per HPF and number of mitotic figures per HPF. S-DNA histogram area was measured by CAS 200 system in arbitrary units. Single strand-DNA mAb area was measured in digital images by Optimas 6.5 and ss-DNA mAb density was measured in arbitrary units of photomultiplier scanner.

From: Rapid in vivo Taxotere quantitative chemosensitivity response by 4.23 Tesla sodium MRI and histo-immunostaining features in N-Methyl-N-Nitrosourea induced breast tumors in rats

Pre-drug treated@ Post-drug treated# Sodium MRI signal intensityd Histology (in HPF) A.I. (KI-67) P.I. (in HPF) S-DNA (histogram) ss-DNA mAb (density units)
Tumor feature IR SQ (CAS)
Control (n = 41) 4.25 ± 1.0 5.27 ± 2.0 - - - - -
Sham (n = 40) 4.30 ± 0.2 5.38 ± 1.0      
Tumor area(mm2;n = 16)@ 4.56 ± 0.30 4.67 ± 0.30 4.49 ± 0.2 - - 3.6 ± 0.2 4.2 ± 0.2
Tumor area(mm2;n = 3)# 4.60 ± 0.29 4.61 ± 0.26 4.42 ± 0.3 - - 3.5 ± 0.3 4.3 ± 0.3
IC/EC space@ 60–70   65–76 - - - -
IC/EC space# 80–95   60–70 - - - -
Necrosis*(squares)@ gray   56 ± 32 - 270 M-DNA -
Necrosis*(squares)# bright   50 ± 23 - 120 M-DNA -
Viable**(squares)@ dark   65 ± 21 - - - -
Viable**(squares)# dark   76 ± 11 - - - -
Apoptosis***(nuclei)@ bright   40 ± 12 150 - S-DNA 159 ± 12
Apoptosis***(nuclei)# bright   50 ± 13 160 - M or S-DNA 129 ± 20
Cyst****(size in μm)@ gray   120 ± 29 - - - -
Cyst****(size in μm)# gray   80 ± 22 - - - -