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Figure 4 | Cancer Cell International

Figure 4

From: Establishment of a doxycycline-regulated cell line with inducible, doubly-stable expression of the wild-type p53 gene from p53-deleted hepatocellular carcinoma cells

Figure 4

Selection of the optimal clone under Dox control. For each of the hygromycin-resistant clones, cells were grown for 24 h in the presence (Dox+) or absence (Dox-) of 1 μg/ml Dox. The expression of p53 protein was analyzed by Western blotting. Lanes 1, 2: 13T (Dox+, Dox-); Lanes 3, 4: 15T (Dox+, Dox-); Lanes 5, 6: 23T (Dox+, Dox-).

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Cancer Cell International

ISSN: 1475-2867

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