Figure 1From: Epigenetic inactivation and aberrant transcription of CSMD1 in squamous cell carcinoma cell linesPositions of the amplicons used for COBRA and bisulfite sequencing relative to CSMD1's first exon and the CpG island. Amplicon 1 extends from -395 to -112 bp, amplicon 2 from +175 to +396 bp, and amplicon 3 from +398 to +718 bp relative to the first base of the transcript. The region between amplicons 1 and 2 is so GC rich that no workable PCR primers could be designed to amplify it after bisulfite conversion. Transcription of CSMD1 is from left to right in the figure.Back to article page