MIA mRNA expression and serum levels. A: MIA mRNA values in normal pancreatic tissues and pancreatic ductal adenocarcinoma (cancer) tissues by real-time quantitative polymerase chain reaction, as described in the Methods section. Values were normalized to housekeeping genes (cyclophilin B and hypoxanthine guanine phosphoribosyltransferase). B: ELISA was carried out as described in the Methods section. Fifty pancreatic cancer sera samples and 14 healthy donor sera samples were analyzed. The horizontal lines represent the mean expression levels.