Localization of overexpressed aurora B-GFP in an interphase NRK cell. Spinning disk laser confocal microscopic images of living NRK cells overexpressing aurora B-GFP in interphase cells. The phase-contrast (a, b) and corresponding fluorescence images (a', b') show the distribution of overexpressed aurora B-GFP (a') or GFP alone (b'). Overexpressed aurora B-GFP is preferentially associated with nucleus (a', arrowhead) and the cortex (a', arrows), while GFP alone is diffusely distributed in the cell. Bar, 10 μm.