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Figure 5 | Cancer Cell International

Figure 5

From: Downregulation of calcineurin activity in cervical carcinoma

Figure 5

NFAT-DNA-binding activity in normal and cervical cancer nuclear extracts. Briefly 100 μg of nuclear extracts was incubated with 32P-labelled NFATc oligonucleotide and Electrophoretic mobility shift assay was performed as described in Material and methods. Lane one contains free probe without nuclear extract and lane 2 contains 100-fold excess of unlabelleled NFAT oligonucleotide as a specific competitor. Specific NFAT- DNA complexes are indicated by arrow.

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