Undifferentiated ES cells exhibit extreme mobility and neuralized ES cells exhibit selective migration toward Glioma Cells and Conditioned Medium. A-C, Cell counts were made after a 4 hours incubation with either Unconditioned Medium (UM; DMEM and FBS alone), Conditioned Medium or Glioma Cells in the bottom well, for all three glioma cell lines, C6, N1321, and U87 (as indicated). On Day 0 (undifferentiated ES cells) and on Day 4, large numbers of stem cells migrated toward the bottom well with no significant differences in cell counts, regardless of the content of the lower well (Ai-Ci). However, neuralized ES cells obtained at Day 8 of the induction protocol exhibited selective migration when Conditioned Medium or Glioma cells were placed in the bottom well for all three glioma cell lines, C6, N1321, and U87, respectively (Aii-Cii). At Day 8, significance was established at p < 0.05 (*) or p < 0.01 (**) using One-Way ANOVA and a post hoc Dunnet Test.