|
Stages of ES Cell Induction and Experimental Condition
| |
Days 0, 4, 8 and Glioma Cells
|
Days 0, 4, 8 and Conditioned Medium
|
Day 8 and rSCF
|
Days 0, 4, 8 and Negative Control 1*
|
Days 0, 4, 8 and Negative Control 2*
|
|---|
|
Top Well
| | | | | | |
| |
DMEM
|
X
|
X
|
X
|
X
|
X
|
| |
Stem Cells
|
X
|
X
|
X
| | |
|
Bottom Well
| | | | | | |
| |
DMEM
|
X
|
X
|
X
|
X
|
X
|
| |
FBS
|
X
|
X
|
X
|
X
|
X
|
| |
Glioma Cells
|
X
| | |
X
| |
| |
Factors Secreted by Tumor Cells
|
X
|
X
| |
X
| |
| |
rSCF
| | |
X
| | |
- * See Methods
- Unconditioned Medium = DMEM and FBS
- Abbreviations: rSCF, recombinant Stem Cell Factor; DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum
