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Table 1 Contents of Top and Bottom Wells for Migration Experiments

From: Selective migration of neuralized embryonic stem cells to stem cell factor and media conditioned by glioma cell lines

Stages of ES Cell Induction and Experimental Condition   Days 0, 4, 8 and Glioma Cells Days 0, 4, 8 and Conditioned Medium Day 8 and rSCF Days 0, 4, 8 and Negative Control 1* Days 0, 4, 8 and Negative Control 2*
Top Well       
  DMEM X X X X X
  Stem Cells X X X   
Bottom Well       
  DMEM X X X X X
  FBS X X X X X
  Glioma Cells X    X  
  Factors Secreted by Tumor Cells X X   X  
  rSCF    X   
  1. * See Methods
  2. Unconditioned Medium = DMEM and FBS
  3. Abbreviations: rSCF, recombinant Stem Cell Factor; DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum