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Table 1 Contents of Top and Bottom Wells for Migration Experiments

From: Selective migration of neuralized embryonic stem cells to stem cell factor and media conditioned by glioma cell lines

Stages of ES Cell Induction and Experimental Condition

 

Days 0, 4, 8 and Glioma Cells

Days 0, 4, 8 and Conditioned Medium

Day 8 and rSCF

Days 0, 4, 8 and Negative Control 1*

Days 0, 4, 8 and Negative Control 2*

Top Well

      
 

DMEM

X

X

X

X

X

 

Stem Cells

X

X

X

  

Bottom Well

      
 

DMEM

X

X

X

X

X

 

FBS

X

X

X

X

X

 

Glioma Cells

X

  

X

 
 

Factors Secreted by Tumor Cells

X

X

 

X

 
 

rSCF

  

X

  
  1. * See Methods
  2. Unconditioned Medium = DMEM and FBS
  3. Abbreviations: rSCF, recombinant Stem Cell Factor; DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum