Stages of ES Cell Induction and Experimental Condition
| |
Days 0, 4, 8 and Glioma Cells
|
Days 0, 4, 8 and Conditioned Medium
|
Day 8 and rSCF
|
Days 0, 4, 8 and Negative Control 1*
|
Days 0, 4, 8 and Negative Control 2*
|
---|
Top Well
| | | | | | |
|
DMEM
|
X
|
X
|
X
|
X
|
X
|
|
Stem Cells
|
X
|
X
|
X
| | |
Bottom Well
| | | | | | |
|
DMEM
|
X
|
X
|
X
|
X
|
X
|
|
FBS
|
X
|
X
|
X
|
X
|
X
|
|
Glioma Cells
|
X
| | |
X
| |
|
Factors Secreted by Tumor Cells
|
X
|
X
| |
X
| |
|
rSCF
| | |
X
| | |
- * See Methods
- Unconditioned Medium = DMEM and FBS
- Abbreviations: rSCF, recombinant Stem Cell Factor; DMEM, Dulbecco's modified eagle medium; FBS, fetal bovine serum