RT-PCR analysis of ILK expression in HOSCCs. RT-PCR was performed using specific primers co-amplifying ILK1 and ILK2. PCR products in the HOSCC cell lines WHCO1, WHCO3, WHCO5, WHCO6 and SNO separated on 2% agarose gels and stained with ethidium bromide. (A) An ILK band of 1360 bp was amplified across all five cell lines. 1 kb ladder in lane 1.