Figure 7
Effect of EGF on ILK expression over time. (A) Serum starved cells were treated with 10 ng/ml EGF for 0, 0.5, 1, 3 and 6 hours respectively and membrane associated fractions immunoblotted as before with a anti-ILK antibody (1:1500) in the WHCO1, WHCO3, WHCO5, WHCO6 and SNO cell lines. (B) Densitometric analysis of the expression levels of ILK in the HOSCCs demonstrated an increased trend in ILK expression, although variations in expression were noted at certain instances.