Figure 8
Effect of TGFβ1 on ILK expression over time. (A) Serum starved cells were treated with 10 ng/ml TGFβ1 for 0, 0.5, 1, 3 and 6 hours respectively. Membrane-associated fractions were resolved on 10% SDS-PAGE gels, blotted onto a nitrocellulose filter and probed with an anti-ILK antibody (1:1500) in the WHCO1, WHCO3, WHCO5, WHCO6 and SNO cell lines. (B) Densitometric analysis revealed that although expression levels of ILK were generally increased across the five HOSCC cell lines, fluctuations were observed in the WHCO1 and SNO cell lines.