Figure 4

HPV 16 transfection did not affect CAL27 adhesion in vitro. The presence and expression of HPV 16 did not affect CAL27 adhesion, as measured by standard 30-minute adhesion assays (A) (n = 32, p = .27). The presence and expression of HPV16 did, however, affect CAL27 adhesion in the modified adhesion assay, increasing adhesion by 43% in the absence of serum and 86% in the presence of serum in three separate, independent trials (n = 96, p < 0.01). The presence of HPV16 did not affect CAL27 adhesion in standard adhesion assays on naked plastic (NP), or wells coated with bovine serum albumin (BSA), but did increase CAL27 adhesion on fibronectin (FN) by 58% (B) (n = 24, p < 0.05).