Figure 3

Concentration of GAPDH transcripts in HepG2 cells treated with antisense primers. HepG2 cells were cultured in absence and in presence of S-ODN1 and S-ODN2 at concentrations of 1 μM or 2 μM for 24 h. & 48 h. as described in legend for fig(2). Purified cellular RNA from 2.5 × 10⁵ cells was RT-PCR amplified using the Taqman EZ-RT-PCR kit as in materials and methods. Fluorescent-labeled amplicons from different samples was calculated for each sample from standard GAPDH RNA provided with kit, the concentrations of GAPDH transcripts throughout the culture were plotted as lines.