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Figure 5 | Cancer Cell International

Figure 5

From: "Nutritional and chemopreventive anti-cancer agents up-regulate expression of p27Kip1, a cyclin-dependent kinase inhibitor, in mouse JB6 epidermal and human MCF7, MDA-MB-321 and AU565 breast cancer cells"

Figure 5

Inhibition of certain receptor protein tyrosine kinases (RPTKs) up-regulates the activity of -575 p27 (5'-untranslated region (5'UTR) of p27 gene). (a) MDA-MB-231, (b) MCF7, (c) AU565, and (d) JB6 cells were transfected with -575 p27 (p27-5'UTR) and then exposed to inhibitors of RPTKs for 24 hours. The following inhibitors were used: AG9 (2.71 μM), AG18 (2.69 μM), AG1478 (1.58 μM), and PD153035 (1.39 μM) to inhibit epidermal growth factor receptor (EGFR); AG30 (2.44 μM) to inhibit c-ErbB; AG1295 (2.13 μM) to inhibit platelet-derived growth factor receptor (PDGFR); IGF-1R inhibitor PPP (1.21 μM), AG1024 (1.64 μM), and AGL2263 (1.55 μM) to inhibit insulin receptors (IR) and type 1 insulin-like growth factor receptors (IGF-1R). All assays were performed in triplicates and the transfection experiments were repeated three times.

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