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Table 2 Sequence analysis of PDGFRα exons 12 and 18 from eight primary cervical cancer cell lines.

From: Expression of platelet derived growth factor family members and the potential role of imatinib mesylate for cervical cancer

CELL LINE EXON 12 EXON 18
  localization change position amino acid localization change position amino acid
ViBo Exon G>A CCA >CCG Codon 567 Pro > Pro Intron C >G IVS18 +21 ---
Exon G>A CCA >CCG Codon 577 Pro > Pro Intron A >G IVS18 +25 ---
Exon A>G TCA >TCG Codon 584 Ser > Ser -----
Intron A> G IVS12 +17 ---- -----
Intron T> C IVS12 +35 ---- -----
ViPa WT Exon C >T GTC>GTT Codon 824 Val > Val
HeLa WT Exon C >T GTC>GTT Codon 824 Val > Val
CaLo WT Exon C >T GTC>GTT Codon 824 Val > Val
INBL WT Exon C >T GTC>GTT Codon 824 Val > Val
C33 Exon G >A GAA>AAA Codon 571 Glu > Lys WT
Caski Exon G >A GAA>AAA Codon 571 Glu > Lys WT
SiHa Exon G >A GAA>AAA Codon 571 Glu > Lys WT
  1. WT: wild type; IVS: intronic variation sequence. We found 7 intronic and 5 exonic variations sequences [RefSeq accession D50017]. None of the intronic variations have been reported: for exon 12 we found, A >G IVS12+17 and T >C IVS12+35, and for exon 18, C >G IVS18+21 and A >G IVS18+25. The exonic changes were: G >A in codon 567 CCA >CCG, Pro>Pro [rs1873778] synonimous; G>A codon 577 CCA >CCG, Pro >Pro (unknown) synonimous; C >T in codon 824 GTC >GTT, Val >Val [rs10015469] synonimous; G >A codon 571 CCA >CCG, Glu > Lys (unknown) not deleterious; and A >G in codon 584 CCA >CCG, Ser > Ser (unknown) synonimous.