Figure 3

Distribution of fragmented extracellular human DNA over various cell compartments of MCF-7 cell culture depending on the time it was present in the culture medium (electrophoresis under denaturing conditions, stage of contact growth inhibition). Agarose blocks stained with ethidium bromide are shown rightward; X-ray patterns of the same blocks after drying, leftward. Numbers above the blocks indicate the time of cell culture incubation with α³²P-labeled extracellular DNA and α-dATP*; numbers to the right of the blocks, markers of molecular weight (Kb); IV, number of experiment. Accumulation of labeled material in the cytoplasm and increase in the linear size of initial fragmented DNA in the interchromosomal space are distinctly seen.