Figure 7
Distribution of individual DNA fragments with lengths of 10.4 and 1.4 kb with blunt ends (designated as Carnegie 20-λp1.4(× 8)Sal GI) either in the presence of salmon sperm DNA (SS DNA) as a competitor or without it and of individual PCR fragments of 230 and 506 bp with tag 3'-ends synthesized from human caspase-5 gene template (designated as 230 and 506) under the same conditions over various cell compartments of MCF-7 cell culture depending on the time they were present in the culture medium (electrophoresis under native conditions, logarithmic stage of cell culture growth). Agarose blocks stained with ethidium bromide are shown rightward; X-ray patterns of the same blocks after drying, leftward. Numbers above the blocks indicate the time of cell culture incubation with α32P-labeled fragments; numbers to the left and right of the blocks, markers of molecular weight (Kb); VI, number of experiment. (A) X-ray pattern of the PCR labeled 506- and 230-bp fragments. (B) The PCR 506- and 230-bp fragments fractionated by agarose electrophoresis, stained with ethidium bromide. Arrow indicates the dimer of 1.4 kb fragment.