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Figure 1 | Cancer Cell International

Figure 1

From: The chemopreventive properties of chlorogenic acid reveal a potential new role for the microsomal glucose-6-phosphate translocase in brain tumor progression

Figure 1

U-87 glioma cells express G6PT and G6Pase-β transcripts with the highest G6PT transcript levels among brain tumor-derived cell lines. Several brain tumor-derived cell lines (glioma : U-87, U-118, U-138; medulloblastoma : DAOY) were cultured until cells reached approximately 90% confluence. Total RNA was extracted and RT-PCR performed in order to generate the cDNA that reflects the gene expression levels of G6PT (380 bp), G6Pase-α (360 bp), and G6Pase-β (236 bp) as described in the methods section. HEP-G2 hepatoma cells were used as positive controls for the presence of all three genes. GAPDH gene expression was used as an internal control for each cell line tested.

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