Figure 2

The G6PT inhibitor chlorogenic acid reduces proMMP-2 secretion in U-87 glioma cells. (A) U-87 glioma cells were serum-starved in the presence of increasing concentrations of CHL and of the green tea catechin EGCg for 18 hrs. Conditioned media was collected and proMMP-2 gelatinolytic activity assessed by zymography as described in the methods section. (B) The extent of proMMP-2 hydrolytic activity was quantified by scan densitometry and is the average ± SEM of three independent experiments. (C) The effect of CHL and EGCg on MT1-MMP functions was also monitored. U-87 cells were cultured in the presence of an exogenous source of proMMP-2 in the presence of 10 μg/ml concanavalin-A, a MT1-MMP-mediated inducer of proMMP-2 activation, and of EGCg or CHL. The extent of proMMP-2 activation was assessed by gelatin zymography.