Figure 4

Chlorogenic acid inhibits sphingosine-1-phosphate-induced U-87 glioma cells migration. (A) Control (Mock) or G6PT-transfected U-87 glioma cells were seeded on gelatin-coated filters and cell migration was assessed in the presence or absence of 100 μM CHL, 1 μM S1P, or a combination of both. (B) Quantification was performed as described in the Methods section and is representative of 6 independent experiments. The average of more than 10 microscopic fields for each experimental condition is shown with SEM.