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Figure 6 | Cancer Cell International

Figure 6

From: The chemopreventive properties of chlorogenic acid reveal a potential new role for the microsomal glucose-6-phosphate translocase in brain tumor progression

Figure 6

Intracellular ATP-depleting agents inhibit proMMP-2 secretion and antagonize S1P-induced ERK phosphorylation in U-87 glioma cells. (A) Serum-starved U-87 glioma cells were treated for 18 hrs with individual concentrations of 2-deoxyglucose or 5-thioglucose, two well established ATP-depleting agents [61]. Conditioned media were collected and the hydrolytic activity of proMMP-2 was assessed using gelatin-zymography as described in the Methods section. (B) The extent of proMMP-2 hydrolytic activity was quantified by scan densitometry in 5-TG- (black circles) and 2-DG- (white circles) treated cells, and is the average ± SEM of three independent experiments. (C) Serum-starved U-87 glioma cells were pretreated for 30 minutes with 100 mM 2-deoxyglucose (2-DG) or 30 mM 5-thioglucose (5-TG). One μM S1P was then added to the media for 15 seconds. Cells were then rapidly harvested and lysates prepared as described in the Methods section.

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