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Figure 2 | Cancer Cell International

Figure 2

From: Selective cytotoxicity of Pancratistatin-related natural Amaryllidaceae alkaloids: evaluation of the activity of two new compounds

Figure 2

A. Dose-response curve for Jurkat cells treated with various concentrations of either AMD4 or AMD5 for 48 hrs. Cells were stained with cell-permeable Hoechst 33342 dye; brightly stained and condensed nuclei were considered to be apoptotic while non-bright, smooth nuclei were considered healthy (non-apoptotic). The degree of apoptosis was calculated by the number of apoptotic cells counted over the total number of cells visible and displayed as a percentage. A minimum of 5 fields with at least 100 cells per field were counted and tabulated using Microsoft Excel software; values that are statistically significant to p < 0.05 are indicated with an asterisk. Micromolar is represented as uM in this figure only. B. Time Course: A measurement of the degree of apoptosis induced in Jurkat cells treated with 10 μM of either AMD4 or AMD5 over 72 hours. Following treatment, Jurkat cells were incubated with cell-permeable Hoechst 33342 dye; brightly stained condensed nuclei were considered to be apoptotic while non-bright, smooth nuclei were considered healthy. The degree of apoptosis was calculated by the number of apoptotic cells counted over the total number of cells visible displayed as a percentage. A minimum of 5 fields with at least 100 cells per field were counted and tabulated using Microsoft Excel software; values that are statistically significant to p < 0.05 are indicated with an asterisk.

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