Figure 4

TUNEL Assay: Jurkat cells were treated with AMD5 at 10 μM for 24 hrs. Following treatment, Jurkat cells were fixed and immuno-stained with anti-BdrU antibody to observe the extent of DNA damage as described in the Materials and Methods section. The level of staining indicates the degree of DNA damage induced by treatment, where more positively stained cells are in the final stages of apoptosis. Magnification: 200×