Figure 3

Suppression of T98G cell motility and invasion by overexpression of HCOL1A1. Mock cells (Mock) and HCOL1A1-transfected cells (HCOL1A1-I and HCOL1A1-II) were added to a transwell chamber and the cell motility was evaluated. (a) The migrated cells were stained and photographed under the microscope at × 100 magnification. (b) The number of migrated cells was counted, and the results represent a percentage of Mock cells. Each value is a mean ± SD (n = 4). *, P < 0.001, when tested against the Mock. Mock cells (Mock) and HCOL1A1-transfected cells (HCOL1A1-I and HCOL1A1-II) were added to a transwell chamber coated with Matrigel and the cell invasion was evaluated. (c) The invaded cells were stained and photographed under a microscope at × 100 magnification. (d) The number of invaded cells was counted, and the results are expressed as a percentage of Mock cells. Each value is a mean ± SD (n = 4). *, P < 0.001, **, P < 0.01 when tested against the Mock. The cell invasion into an in vitro invasion model was evaluated using reconstituted basement membrane wafers. (e) Mock cells (Mock) and HCOL1A1-transfected cells (HCOL1A1-I) were plated onto Matrigel wafers. On days 3 and 7 after plating, Matrigel wafers and adherent cells were fixed, and sections were stained with hematoxylin and eosin. Magnification, × 200.