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Figure 1 | Cancer Cell International

Figure 1

From: HIF-2α downregulation in the absence of functional VHL is not sufficient for renal cell differentiation

Figure 1

Successful knockdown of HIF-2α in both 786-O and A498 renal cell lines. (A) Lysates from 786-O cells stably transfected with VHL (VHL +), parental 786-O cells (786-O), and 786-O cells infected with an empty retrovirus (pSuperRetro) or with retroviruses encoding single HIF-2α shRNAs (HIF2α shRNA 1; HIF2α shRNA 2) or doubly infected with both shRNAs (HIF2α shRNA 1+2) were immunoblotted for HIF-2α (top panel), GLUT1 (upper middle panel) and VHL (lower middle panel). Bottom panel is an alpha tubulin immunoblot to demonstrate equal protein loading (25 μg) in each lane. (B) Lysates from A498 cells stably transfected with VHL (VHL +), parental A498 cells (A498), and A498 cells infected with an empty retrovirus (pSuperRetro) or doubly infected with both HIF-2α shRNAs (HIF2α shRNA 1+2) were immunoblotted as in (A).

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