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Figure 2 | Cancer Cell International

Figure 2

From: HIF-2α downregulation in the absence of functional VHL is not sufficient for renal cell differentiation

Figure 2

Knockdown of HIF-2α mediates cell cycle arrest on collagen I. (A) Parental 786-O cells (786-O), 786-O cells stably transfected with VHL (VHL +), and 786-O cells infected with an empty retrovirus (pSuperRetro) or doubly infected with both HIF-2α shRNAs (HIF2α shRNA 1+2) were grown for 2 weeks after attaining confluence on gels of polymerized collagen I. Flow cytometry was performed with a FACScan instrument. Percentages of cells in S phase are shown for each cell line. (B) FACS analysis was performed as in (A) using A498 cell lines. (C) Graphical representation of the FACS analysis in (B) with arrows highlighting cells with aneuploid DNA content. (D) Complete cell cycle profiles of 786-O and A498 cell lines (diploid cells only) from FACS analyses presented in (A), (B), and (C).

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Cancer Cell International

ISSN: 1475-2867

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