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Figure 4 | Cancer Cell International

Figure 4

From: HIF-2α downregulation in the absence of functional VHL is not sufficient for renal cell differentiation

Figure 4

Knockdown of HIF-2α has a partial effect on downregulation of α5 and β1 integrins. (A) Lysates from 786-O cells stably transfected with VHL (VHL +), parental 786-O cells (786-O), and 786-O cells infected with an empty retrovirus (pSuperRetro) or doubly infected with both HIF-2α shRNAs (HIF2α shRNA 1+2) were immunoblotted for α5 integrin (top panel) and β1 integrin (middle panel). Bottom panel is an alpha tubulin immunoblot to demonstrate equal protein loading (25 μg) in each lane. (B) Integrin immunoblotting was performed as in (A) using A498 cell lines. (C) Integrin immunoblotting was performed as in (A), except cells were grown on thin layers of collagen I.

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