Comparison of percentage of methylation across 5 of the tested CpG positions in vitro and in vivo. Each data point is the average of methylation percentage for each CpG position in three experiments. Y-axis indicates the percentage of methylation and the samples are indicated on the x-axis. The location of CpG positions relative to the gene start site and to each other is shown in Figure 5. The results of 5 CpG positions are shown to represent the methylation percentage in the four genes across the samples. There was a significant decrease (p < 0.001) in methylation quantity for each CpG position after decitabine treatment both in vitro and in vivo for the four genes in all sample but not in the NHOst (normal human osteoblasts). p-values were calculated by comparing the percentage of methylation for each individual CpG position in control cells with the same CpG position in the treated cells using student t-test and they all resulted in p < 0.001.