Figure 8
From: Base excision repair of ionizing radiation-induced DNA damage in G1 and G2 cell cycle phases
The percentage cleavage of oligonucleotide substrate containing DHU lesion. The radiolabeled oligonucleotide substrate was incubated with cell extracts prepared from either unirradiated or 3 Gy irradiated cells collected at various time points. The reaction products were separated on polyacrylamide gel and the incised fragments were quantified to calculate the percentage cleavage at the DHU site.