Figure 4

Epifluorescent micrographs of epithelial cell of primary culture of breast cancer tissue. (Fig. 4A) Bright aggregations of integrin alphavbeta3 immunofluorescence – indicating integrin clustering – were observed along the periphery of the ventral surface of the cell (arrow), while some of them were formed at the leading edge of the advancing lamellipodium (double arrows). Less bright fine granular integrin alphavbeta3 immunofluorescence was also observed along the cell surface (arrowheads). (Fig. 4B) F-actin cytoskeleton, as visualized by rhodamine-conjucated phalloidin, appeared well developed, constituted by numerous thick stress fibers, which were arranged towards the integrin alphavbeta3 immunolocalizations, as demonstrated also in the merged images (Fig. 4C). [750×]