Figure 7

Representative Western immunoblots for integrin beta3 subunit and actin on whole cell extracts of MDA-MB-435 cell line (lane 1), normal breast tissue primary culture cells (lane 2) and primary culture cells of infiltrating breast carcinomas (grade II) (lanes 3, 4, 5). Equal amounts of protein (40 μg) were loaded from each sample and subjected to Western blot analysis. Integrin beta3 subunit was not detected in normal breast tissue cells, while differential expression of beta3 was observed in breast cancer cell samples. In the latter, a higher expression of actin was also observed in comparison with that of normal breast tissue cells.