Gene expression profiling of leukemic cells and primary thymocytes predicts a signature for apoptotic sensitivity to glucocorticoids

Table 3 Conserved gene expression signature for GC-sensitivity in thymocytes and multiple leukemias

GC-response			Sen.	Sen.	Sen.	Sen.	Sen.	Sen.	Sen.	Sen.	Resen.	Res.
Mouse vs. Human			Mouse	Ped.	Ped.	Ped.	Adult	Ped.	Ped.	Ped.	Ped.	Ped.
Cell lineage			T-cell	T-cell	T-cell	T-cell	B-cell	B-cell	Myeloid	T-cell	T-cell	T-cell
Type of cell			Thymocyte	ALL	ALL	ALL	ALL	ALL	AML	ALL	ALL	ALL
Name	Description	Opp. R	C57/BL6 Dx	C7–14 Dx	C7–14 Z	C1–6 Dx	RS4 Dx	SUP Dx	Kas Dx	C1–15 Z	C1–15 Dx+F	C1–15 Dx
BCL2L11	BCL2-like 11 (apoptosis facilitator)		1.8	3.1	14.5	5.9	1.2	3.1	5.1	1.3	1.8	*
CDC6	CDC6 cell division cycle 6 homolog (S. cerevisiae)		-1.2	-1.3	-1.2	-1.5	-1.7	-2.9	-4.7	-1.3	-1.3	-1.3
DDIT4	DNA-damage-inducible transcript 4		2.9	4.4	3.4	2.5	5.5	4.6	22.4	1.2	4.4	1.5
DSCR1	Down syndrome critical region gene 1		1.3	5.0	8.2	4.8	1.5	7.7	7.9	1.4	1.6	*
FKBP5	FK506 binding protein 5		1.7	6.6	11.2	4.4	7.0	21.4	13.7	3.8	2.9	2.8
NFKBIA	NFκB inhibitor, alpha		1.5	3.0	3.4	2.8	1.9	2.5	5.5	1.9	1.5	1.3
OGT	O-linked N-acetylglucosamine (GlcNAc) transferase	◯	-1.5	1.3	1.7	2.0	2.0	1.3	2.1	1.6	1.3	*
PA2G4	proliferation-associated 2G4, 38 kDa	◯	1.2	-1.7	-1.9	-1.7	-1.3	-2.5	-3.5	-1.3	-1.2	*
PRG1	proteoglycan 1, secretory granule		1.6	2.6	4.5	3.2	2.7	1.5	2.7	1.2	1.9	1.6
TXNIP	thioredoxin interacting protein		2.6	2.8	3.4	3.7	10.0	7.7	2.1	1.4	2.0	1.2

To obtain genes regulated by GCs in a conserved manner in both pediatric (Ped.) and adult leukemias, the 27 genes regulated in the same sense among all naturally GC-sensitive (Sen.) and resistant-restored-to-sensitive (Resen.) human leukemias (Table 2) were compared to mouse thymocytes (C57/BL6 Dx). This analysis identified 10 genes, 2 of which were regulated in an opposite sense regulation between human and mouse indicated by (Opp. R, symbol, closed circle). This list was also compared to Dex-treated resistant (Res.) CEM-C1–15 cells (C1–15 Dx). Bold type indicates statistically significant regulation p ≤ 0.05 between means of vehicle vs. GC-treated. Symbol, asterisk = gene "absent" by selection criteria.

ISSN: 1475-2867