Figure 8

Transcriptional potency of the GR is enhanced upon restoration of Dex-sensitivity to CEM-C1-15 cells. CEM-C1-15 cells were electroporated with a plasmid expressing three GREs in tandem fused to a SEAP reporter. Cells were subsequently pretreated with vehicle control, FSK (F), U0126 (U) plus SP600125 (SP), U0126 plus (ip), or combinations of these treatments for 6 hours before adding Dex for an additional 24 hours with subsequent evaluation of SEAP activity. Drug treatment alone was subtracted as background and shown is the Dex effect on each treatment. Error bars: 1 standard deviation, average of 3 independent replicates; p-value is based on a t-test for matched drug treatments ± Dex, n = 2–3 for various combinations of treatments.