Pathway interactions between MAPKs, mTOR, PKA, and the glucocorticoid receptor in lymphoid cells

Table 1 Forskolin and Rapamycin sensitize CEM-C1-15 cells to Dex.

Dex resistant CEM-C1-15	C	Dex	F	F+Dex	R	R+Dex
Viable Cells/ml (× 10e5)	19	16	7.1	1.4	3.6	1.7
Significance (VC/ml, C vs Rx) p =		0.11		0.01		0.004
% of Drug matched control		84		20		47
% Culture viability	99	98	90	38	97	87
Dex sensitive CEM-C7-14
Viable Cells/ml (× 10e5)	14	3.2
Significance (VC/ml, C vs Rx) p =		0.002
% of Drug matched control		23
% Culture viability	98	54

Cells were plated in triplicate wells at 1 × 10⁵ viable cells/ml and treated with FSK or rapamycin (C = vehicle, F =FSK, R = rapamycin). Dex was added within 5 minutes of FSK addition and 6 hours after addition of rapamycin. Cells were evaluated by trypan blue exclusion at 48 hours for FSK and 72 hours for rapamycin and Dex alone treatments; p-value indicates results of a paired t-test on viable cell numbers for each drug matched sample. To evaluate the Dex effect for each treatment, viable cell numbers for each drug and drug plus Dex were compared and expressed as % of drug matched control. Percent culture viability indicates the percentage of cells able to exclude trypan blue dye (viable cells) at the time of evaluation. Dex-sensitive CEM-C7-14 is included as a positive control for Dex sensitivity, n = 1–3.

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