Ultraviolet-A radiation induces changes in cyclin G gene expression in mouse melanoma B16-F1 cells

Pastila, Riikka; Leszczynski, Dariusz

doi:10.1186/1475-2867-7-7

Cancer Cell International

Table 1 Differentially expressed genes after UVA dose of 8 J/cm²

From: Ultraviolet-A radiation induces changes in cyclin G gene expression in mouse melanoma B16-F1 cells

Gene family [Swiss prot Accession #]	No. of arrays^a	Control^b± SD	UVA exposed^c± SD	t-test	Ratio^d	Function
Stress induced
Heat shock 86-kDa protein (HSP86) [P07901]	3 ↑	0,32 ± 0,23	1,04 ± 0,44	0,43	3,23	Belongs to HSP90 family; cytoplasmic molecular chaperone regulating the correct folding in the heat induced conformational changes.
Heat shock cognate 71-kDa (HSC70; HSP73), mouse homolog of human [P11142]	2 ↑ 1 ↓	0,15 ± 0,12	0,27 ± 0,18	0,39	1,81	Belongs to HSP70 family; molecular chaperone regulating the correct folding; found in melanoma cell lines [33].
Alpha crystallin B-subunit, mouse homolog of human [P02511]	3 ↑	0,07 ± 0,03	0,21± 0,07	0,05	3,26	Belongs to HSP20 family; found in mammalian transparent lens in eye induced by stress [34,35].
Oxidative Stress
Oxidative stress-induced protein (OSI) [Q64337]	2 ↑ 1 ↓	0,16 ± 0,14	0,31± 0,15	0,27	1,92	Regulates metabolic responses to oxidative stress, were also induced [36]
glutathione S-transferase mu2 (GSTM2); [P15626]	3 ↑ 1 ↓	0,07 ± 0,04	0,14 ± 0,09	0,23	2,09	Belongs to the GST superfamily; prevents the toxic injuries; expressed in human melanoma cells [37] and in keratinocytes from human squamous cell carcinoma [38].
Cell cycle control
cyclin G [O54779; P51945]	5 ↑	0,13 ± 0,04	0,65 ± 0,36	0,03	4,85	Contributes to G2/M arrest in response to DNA damage; a transcriptional target of the p53.
Angiogenesis
Vascular endothelial growth factor (VEGF) [Q00731]	4 ↑	0,07 ± 0,03	0,22 ± 0,08	0,03	2,94	Promotes endothelial cell proliferation and migration in angiogenesis; permeabilizes the blood vessels; expression regulated by UVB in keratinocytes and fibroblasts [39,40].
Skeleton & Motility protein
G-actin cytoplasmic [P02571; P14104]	3 ↓	1,02 ± 0,37	0,47 ± 0,18	0,10	0,47	Exists in all eukaryotic cells as a component of the cytoskeleton mediating cell motility. Polymerization leads to formation of filamentous F-actin.
non-muscle cofilin 1 (CFL1) [P18760]	3 ↓	0,40 ± 0,10	0,17 ± 0,01	0,05	0,41	Controls reversibly actin depolymerization from F-actin to G-actin causing an increase in the G-actin pool.

^aThe number of arrays (out of five), in which differences in the gene expression were observed.; The arrows indicate the upregulation or downregulation of the gene. In the remaining arrays no difference was observed.
^bThe average of the normalized gene intensity in control cells ± standard deviation;
^cThe average of the normalized gene intensity in UVA exposed cells ± standard deviation.
^dRatio of UVA exposed genes versus control genes.

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ISSN: 1475-2867

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