Skip to main content

Table 1 Differentially expressed genes after UVA dose of 8 J/cm2

From: Ultraviolet-A radiation induces changes in cyclin G gene expression in mouse melanoma B16-F1 cells

Gene family [Swiss prot Accession #] No. of arraysa Controlb± SD UVA exposedc± SD t-test Ratiod Function
Stress induced
Heat shock 86-kDa protein (HSP86) [P07901] 3 ↑ 0,32 ± 0,23 1,04 ± 0,44 0,43 3,23 Belongs to HSP90 family; cytoplasmic molecular chaperone regulating the correct folding in the heat induced conformational changes.
Heat shock cognate 71-kDa (HSC70; HSP73), mouse homolog of human [P11142] 2 ↑
1 ↓
0,15 ± 0,12 0,27 ± 0,18 0,39 1,81 Belongs to HSP70 family; molecular chaperone regulating the correct folding; found in melanoma cell lines [33].
Alpha crystallin B-subunit, mouse homolog of human [P02511] 3 ↑ 0,07 ± 0,03 0,21± 0,07 0,05 3,26 Belongs to HSP20 family; found in mammalian transparent lens in eye induced by stress [34,35].
Oxidative Stress
Oxidative stress-induced protein (OSI) [Q64337] 2 ↑
1 ↓
0,16 ± 0,14 0,31± 0,15 0,27 1,92 Regulates metabolic responses to oxidative stress, were also induced [36]
glutathione S-transferase mu2 (GSTM2); [P15626] 3 ↑
1 ↓
0,07 ± 0,04 0,14 ± 0,09 0,23 2,09 Belongs to the GST superfamily; prevents the toxic injuries; expressed in human melanoma cells [37] and in keratinocytes from human squamous cell carcinoma [38].
Cell cycle control
cyclin G [O54779; P51945] 5 ↑ 0,13 ± 0,04 0,65 ± 0,36 0,03 4,85 Contributes to G2/M arrest in response to DNA damage; a transcriptional target of the p53.
Angiogenesis
Vascular endothelial growth factor (VEGF) [Q00731] 4 ↑ 0,07 ± 0,03 0,22 ± 0,08 0,03 2,94 Promotes endothelial cell proliferation and migration in angiogenesis; permeabilizes the blood vessels; expression regulated by UVB in keratinocytes and fibroblasts [39,40].
Skeleton & Motility protein
G-actin cytoplasmic [P02571; P14104] 3 ↓ 1,02 ± 0,37 0,47 ± 0,18 0,10 0,47 Exists in all eukaryotic cells as a component of the cytoskeleton mediating cell motility. Polymerization leads to formation of filamentous F-actin.
non-muscle cofilin 1 (CFL1) [P18760] 3 ↓ 0,40 ± 0,10 0,17 ± 0,01 0,05 0,41 Controls reversibly actin depolymerization from F-actin to G-actin causing an increase in the G-actin pool.
  1. aThe number of arrays (out of five), in which differences in the gene expression were observed.; The arrows indicate the upregulation or downregulation of the gene. In the remaining arrays no difference was observed.
  2. bThe average of the normalized gene intensity in control cells ± standard deviation;
  3. cThe average of the normalized gene intensity in UVA exposed cells ± standard deviation.
  4. dRatio of UVA exposed genes versus control genes.