Ewing tumor cells overexpressing EGFR. Cells (EWS TC71 and EWS IOR/CAR) were fixed and stained for EGFR using rabbit polyclonal antibodies, which were visualized with goat Cy3-conjugated secondary antibodies. Images show Cy3 fluorescence (left), DAPI staining of nuclei (middle) and merge (right). Images were recorded by laser scanning microscopy. Bars indicate 10 μM.