Table 1 DNA sequences of all primers used in methylation sensitive PCR

RASSF1A

F 5' GTGTTAACGCGTTGCGTATC 3'

F 5'TTTGGTTGGAGTGTGTTAATGTG 3'

R 5' AACCCCGCGAACTAAAAACGA 3'

R CAAACCCCACAAACTAAAAACAA 3'

Survivin

F 5' GGCGGGAGGATTATAATTTTCG 3'

F 5' GGTGGGAGGATTATAATTTTTG 3'

R 5' CCGCCACCTCTACCAACG 3'

R 5' CCACCACCACCACCTCTAC 3'

DAPK1

F 5'GGATAGTCGGATCGAGTTAACGTC 3'

F 5'GGATAGTTGGATTGAGTTAAYGTC 3'

R 5' CCCTCCCAAACGCCGA 3'

R 5' CAAATCCCTCCCAAACACCAA 3'

APC

F 5' TATTGCGGAGTGCGGGTC 3'

F 5' GTGTTTTATTGTGGAGTGTGGGTT 3'

R 5' TCGACGAACTCCCGACGA 3'

R 5' CCAATCAACAAACTCCCAACAA 3'

RAR-β

F 5' TCGAGAACGCGAGCGATTCG 3'

F 5' TTGAGAATGTGAGTGATTTGA 3'

R 5' GACCAATCCAACCGAAACGA 3'

R 5' AACCAATCCAACAAAACAA 3'

hLMH1

F 5'ACGTAGACGTTTATTAGGGTCGC 3'

F 'TTTTGATGTAGATGTTTTATTAGGGTTGT 3'

R 5'CCTCATCGTAACTACCGCG 3'

R 5'ACCACCTCATCATAACTACCCACA 3'

RB1

F 5'GGGAGTTTCGCGGACGTGAC 3'

F 5'GGGAGTTTTGTGGATGTGAT 3'

R 5'ACGTCGAAACACGCCCCG 3'

R 5'ACATCAAAACAACCCCA 3'

BRCA

F 5'GGTTAATTTAGAGTTTCGAGAGACG 3'

F 5' GGTTAATTTAGAGTTTTGAGAGATG 3'

R 5' TCAACGAACTCACGCCGCGCAATCG 3'

5' TCAACAAACTCACACCACACAATCA 3'

TIMP3

F 5' CGTTTCGTTATTTTTTGTTTTCGGTTTC 3'

F 5' TTTTGTTTTGTTATTTTTTGTTTTTGGTTTT 3'

R 5' CCGAAAACCCCGCCTCG 3'

R 5' CCCCCAAAAACCCCACCTCA 3'

VHL

F 5' TGGAGGATTTTTTTGCGTACGC 3'

F 5' GTTGGAGGATTTTTTTGTGTATGT3'

R 5' GAACCGAACGCCGCGAA 3'

R 5' CCCAAACCAAACACCACAAA 3'

p16

F 5' TTATTAGAGGGTGGGGCGGATCGC 3'

F 5' TTATTAGAGGGTGGGGTGGATTGT 3'

R 5' ACCCCGAACCGCGACCGTAA 3'

F 5' CAACCCCAAACCACAACCATAA 3'

PTEN

F 5' GGTTTTTCGAGGCGTTCG 3'

F 5' TGGTTTTTTGAGGTGTTTG 3'

R 5' CGCCTCACAACGACTCAACT 3'

R 5' TTCCATCATAACTACAACTTCCA 3'

CDH1

F 5' GTGGGCGGGTCGTTAGTTTC 3'

F 5' GGTGGGTGGGTTGTTAGTTTTGT 3'

R 5' CTCACAAATACTTTACAATTCCGACG 3'

R 5' AACTCACAAATCTTTACAATTCCAAC 3'

CASP8

F 5' TAGGGGATTCGGACATTGCGA 3'

F 5' TAGGGGATTTGGAGATTGTGTA 3'

R 5' CGTATATCTACATTCGAAACG 3'

R 5' CCATATATATCTACATTCAAAACAA 3'

hTERTc

F 5' GACGTAAAGTTTTTTTCGGACG 3'

F 5' GTAAAGATGTAAAGTTTTTTTTGGATG 3'

R 5' ACCCGATACGCTACCGAACG 3'

R 5' CCACAACCCAATACACTACCA 3'

RASSF1c

F 5' AGTTTGGATTGTCGGTTTCG 3'

F 5' GGAGTTTGGATTGTTGGTTTTG 3'

R 5' TCACAAACCCCACCTACCAC 3'

R 5' CACCCCCAAAAATAACCTCAT 3'