Figure 5

Apoptosis was induced by suppression of H3-K9 MTs, G9A or SUV39H. A. ELISA-based apoptosis analysis shows much higher cytoplasmic histone-associated DNA fragments in the immortalized human bronchoepithelial cells knocked down with G9A or SUV39H compared to the cells siRandom non-targeting control although statistical significance is not reached. B. Apoptosis level seemed not be affected by suppressing any HKMTs in Ras transformed bronchoepithelial cells. The experiments are done in triplicates and repeated twice. Columns, averages; bars, SD.