Figure 3

Expression of CDK inhibitors and pRb family proteins. A: Y79 cells were treated with 5 mM DFMO for 24 and 48 h. Cell lysates (100 μg) were subjected to western blot analysis using the antibodies against p16, p21, p27, and cytochrome c (as a control). Quantification of the bands was performed using the NIH image software, and corrected using the levels of cytochrome c. The data were expressed as %, compared to that observed at 0 h (100%). The experiments were repeated twice and similar results were obtained. A typical result is presented. B: Cell lysates (100 μg) of Y79 cells and Jurkat cells (positive control) were subjected to western blot analysis using the antibody against pRb (upper panel). Y79 cells were treated with 5 mM DFMO for 24 and 48 h. Cell lysates (50 μg) were subjected to western blot analysis using the antibodies against p107 and p130 (middle and lower panels). Signals were visualized using ECL. pp107 and p107 indicate hyperphosphorylated p107 and hypophosphorylated p107, respectively. 1, 2, and 3 indicate phosphorylated forms of p130.