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Table 2 Oligonucleotides used for PCR amplification

From: Inhibition of androgen-independent prostate cancer cell growth is enhanced by combination therapy targeting Hedgehog and ErbB signalling

Gene

Sequence 5'-3'

Size of product (Base Pairs)

AR-f

GACGCTTCTACCAGCTCACC

60

AR-r

GAAAGGATCTTGGGCACTTG

 

β-actin-f

GATGAGATTGGCATGGCTTT

60

β-actin-r

CACCTTCACCGTTCCAGTTT

 

DD3PCA3

GGTGGGAAGGACCTGATGATAG

58

DD3PCA3

GGGCGAGGCTCATCGAT

 

ErbB2-f

CCTCTGACGTCCATCGTCTC

100

ErbB2-r

CGGATCTTCTGCTGCCGTCG

 

EGFR-f

CTTCTTGCAGCGATACAGCTC

439

EGFR-r

ATGCTCCAATAAATTCACTGC

 

GLI1-f

GAAGACCTCTCCAGCTTGGA

306

GLI1-r

GGCTGACAGTATAGGCAGAG

 

GREX-f*

GAGTACCTCTGGAGGACAGA

281

GRINTRON-r*

ATGTCCATTCTTAAGAAACAGGA

 

PSA-f

GGTGACCAAGTTCATGCTGTG

194

PSA-r

GTGTCCTTGATCCACTTCCG

 

PTCH-1-f

CTCCCAAGCAAATGTACGAGCA

150

PTCH-1-r

TGAGTGGAGTTCTGTGCGACAC

 

β2-microglobulin-f

TGAATTCGTATGTGTCTGGGT

247

β2-microglobulin-r

CCTCCATGATGCTGCTTACAT

 
  1. * GREX/GRINTRON = glucocorticoid receptor intron genomic DNA control. Sequence for GRINTRON crosses boundary of glucocorticoid receptor gene intron and exon. No product is formed unless genomic DNA is present.
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Cancer Cell International

ISSN: 1475-2867

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