Figure 4

Effect of 4-HC on the formation of experimental metastases. Melanoma cells were treated in vitro with 500 μM 4-HC for 24 h, and then injected into the tail vein of C57BL/6 mice. Two weeks later, mice were sacrificed and the macroscopic pulmonary tumors were counted. Mice injected with 4-HC-treated cells (triangles) showed a 7-fold decrease in the number of lung tumors compared with the mice that received control B16-F10 cells (squares). ** P = 0.002 (Student's t test with Welch's correction). The pictures on the right show examples of lungs from mice injected with either control or 4-HC-treated cells.